Recombinant Human SCN2A 293 Cell Lysate

• Cat.No.: SCN2A-2030HCL

Specification

• Description: Antigen standard for sodium channel, voltage-gated, type II, alpha subunit (SCN2A), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: SCN2A sodium channel, voltage-gated, type II, alpha subunit [ Homo sapiens ]
• Official Symbol: SCN2A
• Synonyms: HBA; NAC2; BFIC3; BFIS3; BFNIS; HBSCI; EIEE11; HBSCII; Nav1.2; SCN2A1; SCN2A2; Na(v)1.2; sodium channel protein type 2 subunit alphaHBSC II; voltage-gated sodium channel subtype II; sodium channel protein type II subunit alpha; sodium channel protein brain II subunit alpha; voltage-gated sodium channel subunit alpha Nav1.2; sodium channel protein, brain type 2 alpha subunit; sodium channel, voltage-gated, type II, alpha 1 polypeptide; sodium channel, voltage-gated, type II, alpha 2 polypeptide
• Gene ID: 6326
• mRNA Refseq: NM_021007
• Protein Refseq: NP_066287
• MIM: 182390
• UniProt ID: Q99250
• Chromosome Location: 2q24.3
• Pathway: Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; L1CAM interactions, organism-specific biosystem
• Function: voltage-gated sodium channel activity