Recombinant Human SARS, His-tagged

• Cat.No.: SARS-30612TH
• Product Overview: Recombinant full length Human Seryl-tRNA synthetase (amino acids 1-514) with N terminal His tag; 537 amino acids with tag, Predicted MWt 61.2 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 514 amino acids
• Description: This gene belongs to the class II amino-acyl tRNA family. The encoded enzyme catalyzes the transfer of L-serine to tRNA (Ser) and is related to bacterial and yeast counterparts. Multiple alternatively spliced transcript variants have been described but the biological validity of all variants is unknown.
• Conjugation: HIS
• Molecular Weight: 61.200kDa inclusive of tags
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.32% Tris HCl, 0.02% DTT, 10% Glycerol, 0.58% Sodium chloride
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMGSMVLDLDLFRVDKGGDPA LIRETQEKRFKDPGLVDQLVKADSEWRRCRFRADNLNKLK NLCSKTIGEKMKKKEPVGDDESVPENVLSFDDLTADALAN LKVSQIKKVRLLIDEAILKCDAERIKLEAERFENLREIGN LLHPSVPISNDEDVDNKVERIWGDCTVRKKYSHVDLVVMV DGFEGEKGAVVAGSRGYFLKGVLVFLEQALIQYALRTLGS RGYIPIYTPFFMRKEVMQEVAQLSQFDEELYKVIGKGSEK SDDNSYDEKYLIATSEQPIAALHRDEWLRPEDLPIKYAGL STCFRQEVGSHGRDTRGIFRVHQFEKIEQFVYSSPHDNKS WEMFEEMITTAEEFYQSLGIPYHIVNIVSGSLNHAASKKL DLEAWFPGSGAFRELVSCSNCTDYQARRLRIRYGQTKKMM DKVEFVHMLNATMCATTRTICAILENYQTEKGITVPEKLK EFMPPGLQELIPFVKPAPIEQEPSKKQKKQHEGSKKKAAA RDVTLENRLQNMEVTDA
• Sequence Similarities: Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Gene Information

• Gene Name: SARS seryl-tRNA synthetase [ Homo sapiens ]
• Official Symbol: SARS
• Synonyms: SARS; seryl-tRNA synthetase; seryl-tRNA synthetase, cytoplasmic; serine tRNA ligase 1; cytoplasmic; SERS
• Gene ID: 6301
• mRNA Refseq: NM_006513
• Protein Refseq: NP_006504
• MIM: 607529
• Uniprot ID: P49591
• Chromosome Location: 1p13.3
• Pathway: Aminoacyl-tRNA biosynthesis, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, conserved biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, conserved biosystem; Cytosolic tRNA aminoacylation, organism-specific biosystem
• Function: ATP binding; RNA binding; ligase activity; nucleotide binding; serine-tRNA ligase activity