Recombinant Human SAA1 Protein, His-tagged

• Cat.No.: SAA1-6233H
• Product Overview: Recombinant Human SAA1 Protein(Arg19~Tyr122), fused with N-terminal His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: N-His
• Protein length: Arg19~Tyr122
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl, 5% Trehalose.
• Bio-activity: Serum Amyloid A (SAA) is a class of 104 amino acid conservative acute-phase proteins, which is essential in immune-mediated inflammatory processes, like IBD. SAA has dual roles in the inflammatory process, which it can stimulates the pro-inflammatory cytokine expression and promotes the pathogenic differentiation of TH17 cells. In addition, SAA can remove toxic lipids produced during inflammatory responses and membrane debris from dead cells, redirect HDL, and recycle cholesterol for tissue repair. Besides, C Reactive Protein (CRP) has been identified as an interactor of SAA, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human SAA and recombinant human CRP. Briefly, SAA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CRP-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-SAA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37°C. Finally, add 50 μL stop solution to the wells and read at 450 nm immediately. The EC50 for this effect is 5.3 ug/mL.
• Molecular Mass: 12.9kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: >95%
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

Gene Information

• Gene Name: SAA1 serum amyloid A1 [ Homo sapiens ]
• Official Symbol: SAA1
• Synonyms: SAA1; serum amyloid A1; SAA; serum amyloid A protein; PIG4; TP53I4; tumor protein p53 inducible protein 4; SAA2; MGC111216
• Gene ID: 6288
• mRNA Refseq: NM_000331
• Protein Refseq: NP_000322
• MIM: 104750
• UniProt ID: P02735