Recombinant Human S100G, His-tagged

• Cat.No.: S100G-3735H
• Product Overview: Total 87 AA. MW: 10.04 kDa (calculated). N-Terminal His-tag, 9 extra AA (highlighted).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Protein S100-G is a protein that in humans is encoded by the S100G gene. This gene encodes calbindin D9K, a vitamin D-dependent calcium-binding protein. This cytosolic protein belongs to a family of calcium-binding proteins that includes calmodulin, parvalbumin, troponin C, and S100 protein. In the intestine, the protein is vitamin D-dependent and its expression correlates with calcium transport activity. The protein may increase Ca absorption by buffering Ca in the cytoplasm and increase ATP-dependent Ca transport in duodenal basolateral membrane vesicles.
• Form: Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 50mM NaCl, pH 7.5.
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
• Sequences of amino acids: MKHHHHHHAS TK­KSPEELKR IFEKYAAKEG DPDQLSKDEL KLLIQAEFPS LLKGPNTLDD LFQELDKNGD GEVSFEEFQV LVKKISQ
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Gene Information

• Gene Name: S100G S100 calcium binding protein G [ Homo sapiens ]
• Official Symbol: S100G
• Synonyms: S100G; S100 calcium binding protein G; CABP; CABP1; CALB3; CABP9K; MGC138379; protein S100-G; calbindin D9K; OTTHUMP00000022988; S100 calcium-binding protein G; vitamin D-dependent calcium-binding protein, intestinal; calbindin 3, (vitamin D-dependent calcium-binding protein); S100D
• Gene ID: 795
• mRNA Refseq: NM_004057
• Protein Refseq: NP_004048
• MIM: 302020
• UniProt ID: P29377
• Chromosome Location: Xp22.2
• Pathway: Mineral absorption
• Function: calcium ion binding; vitamin D binding