Recombinant Human S100A8/S100A9 Heterodimer Protein, Met1-Glu93 and Thr2-Pro114

• Cat.No.: S100A8/S100A9-54H
• Product Overview: Recombinant human S100A8 (Met1-Glu93) and S100A9 (Thr2-Pro114) Heterodimer protein without tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• ProteinLength: S100A8: Met1-Glu93; S100A9: Thr2-Pro114
• Description: S100A8 (also known as MRP8, Calgranulin A, and CP-10) and S100A9 (also known as MRP14 and Calgranulin B) are pro-inflammatory members of the S100 family of secreted calcium binding proteins. They are up-regulated in neutrophils and monocytes at sites of inflammation (e.g. psoriasis, rheumatoid arthritis, cardiac ischemia) and are present at elevated concentrations in rheumatoid arthritis synovial fluid. The 10 kDa human S100A8 and 14 kDa S100A9 each contain two EF-hand calcium binding motifs. Human S100A8 shares 57% and 61% amino acid (aa) sequence identity with mouse and rat S100A8, respectively. Human S100A9 shares 57% and 62% amino acid sequence identity with mouse and rat S100A9, respectively. S100A8 and S100A9 are noncovalent homodimers that can also noncovalently heterodimerize; in the presence of calcium and zinc, the homodimer and heterodimers will form tetramers. The heterodimer additionally binds and sequesters manganese, thereby restricting the growth of Mn-dependent bacteria. The S100A8/A9 heterodimer exhibits functions beyond those performed by the individual proteins. These include binding to fatty acids such as arachidonic acid and promoting astrocyte proliferation. S100A8, S100A9, and the heterodimer each promote neutrophil infiltration into sites of inflammation and inflammatory cytokine production by monocytes.
• Form: Noncovalently-linked heterodimer
• Bio-activity: Measured by its ability to induce IL-6 secretion by A375 human melanoma cells. The ED50 for this effect is 1.5-6 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 11 kDa (S100A8) & 13 kDa (S100A9)
  SDS-PAGE: 9 kDa (S100A8) & 13 kDa (S100A9), reducing conditions
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• N-terminal Sequence Analysis: Met1 (S100A8) & Thr2 (S100A9)
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
• Reconstitution: Reconstitute at 500 μg/mL in sterile PBS.
• References: 1. Averill, M.M. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:223.
  2. Vogl, T. et al. (2012) Int. J. Mol. Sci. 13:2893.
  3. Siegenthaler, G. et al. (1997) J. Biol. Chem. 272:9371.

Gene Information

• Gene Name: S100A8 S100 calcium binding protein A8 [ Homo sapiens (human) ]
• Official Symbol: S100A8
• Synonyms: S100A8; S100 calcium binding protein A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG; S100-A8; protein S100-A8; calgranulin A; calprotectin L1L subunit; cystic fibrosis antigen; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; urinary stone protein band A; S100 Calcium Binding Protein A8/S100 Calcium Binding Protein A9; S100A8/S100A9; S100A9; S100 calcium binding protein A9; MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387; S100-A9; protein S100-A9; MRP-14; calgranulin B; calprotectin L1H subunit; leukocyte L1 complex heavy chain; migration inhibitory factor-related protein 14
• Gene ID: 6279
• mRNA Refseq: NM_002964
• Protein Refseq: NP_002955
• MIM: 123885
• UniProt ID: P05109