Recombinant Human S100A2, His-tagged

• Cat.No.: S100A2-3725H
• Product Overview: Total 108 AA. MW: 12.36 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Protein S100-A2 is a protein that in humans is encoded by the S100A2 gene. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may have a tumor suppressor function. Chromosomal rearrangements and altered expression of this gene have been implicated in breast cancer.
• Form: Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 50mM NaCl, pH 7.5.
• Purity: 95%, as determined by densitometric image analysis.
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
• Sequences of amino acids: MKHHHHHHAS MMCSSLEQAL AVLVTTFHKY SCQEGDKFKL SKGEMKELLH KELPSFVGEK VDEEGLKKLM GSLDENSDQQ VDFQEYAVFL ALITVMCNDF FQGCPDRP
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
• Pathways: Direct p53 effectors

Gene Information

• Gene Name: S100A2 S100 calcium binding protein A2 [ Homo sapiens ]
• Official Symbol: S100A2
• Synonyms: S100A2; S100 calcium binding protein A2; CAN19; S100L; MGC111539; protein S100-A2; OTTHUMP00000032967; S100 calcium-binding protein A2; Protein S100-A2; Protein S-100L
• Gene ID: 6273
• mRNA Refseq: NM_005978
• Protein Refseq: NP_005969
• MIM: 176993
• UniProt ID: P29034
• Chromosome Location: 1q21
• Function: calcium ion binding