Recombinant Human S100A1 protein, His-tagged

• Cat.No.: S100A1-318H
• Product Overview: Recombinant Human S100A1 protein (Met1-Ser94), fused to His tag at C-terminus, was expressed in E.coli.

Specification

• Description: Protein S100-A1/S100A1 is a Ca2+binding protein of the EF-hand type that belongs to the S100 protein family. S100 proteins consisting of at least 19 members exist as dimers in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell-cycle progression and cell differentiation. S100-A1 can weakly bind calcium but bind zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH 8.0, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 11.4 kDa. The protein migrates as 11 kDa under reducing (R) condition (SDS-PAGE).
• Protein length: 94
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: S100A1
• Official Symbol: S100A1
• Synonyms: S100A1; S100 calcium binding protein A1; S100 calcium binding protein A1 , S100A; protein S100-A1; S100 alpha; S-100 protein alpha chain; S-100 protein subunit alpha; S100 calcium-binding protein A1; S100 protein, alpha polypeptide; S100; S100A; S100-alpha
• Gene ID: 6271
• mRNA Refseq: NM_006271
• Protein Refseq: NP_006262
• MIM: 176940
• UniProt ID: P23297