Recombinant Human RXRB 293 Cell Lysate

• Cat.No.: RXRB-2098HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for retinoid X receptor, beta (RXRB) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: RXRB retinoid X receptor, beta [ Homo sapiens ]
• Official Symbol: RXRB
• Synonyms: RXRB; retinoid X receptor, beta; retinoic acid receptor RXR-beta; H 2RIIBP; NR2B2; nuclear receptor subfamily 2 group B member 2; RCoR 1; retinoid X nuclear receptor beta; MHC class I promoter binding protein; DAUDI6; RCoR-1; H-2RIIBP; MGC1831
• Gene ID: 6257
• mRNA Refseq: NM_021976
• Protein Refseq: NP_068811
• MIM: 180246
• UniProt ID: P28702
• Chromosome Location: 6p21.3
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Non-small cell lung cancer, organism-specific biosystem; Non-small cell lung cancer, conserved biosystem; Nuclear Receptor transcription pathway, organism-specific biosystem
• Function: 9-cis retinoic acid receptor activity; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; metal ion binding; protein binding; protein heterodimerization activity; receptor activity; retinoic acid receptor binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; steroid binding; steroid hormone receptor activity; thyroid hormone receptor binding; thyroid hormone receptor coactivator activity; transcription coactivator activity; transcription regulatory region DNA binding; vitamin D receptor binding; zinc ion binding