Recombinant Human RSPO1 protein, His & T7-tagged

• Cat.No.: RSPO1-1942H
• Product Overview: Recombinant Human RSPO1 aa. (Ser21~Ala135 (Accession # Q2MKA7)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Ser21~Ala135
• Description: This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.5kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 8.9

Gene Information

• Gene Name: RSPO1 R-spondin 1 [ Homo sapiens (human) ]
• Official Symbol: RSPO1
• Synonyms: RSPO1; R-spondin 1; RSPO; CRISTIN3; R-spondin-1; R-spondin homolog; roof plate-specific spondin-1
• Gene ID: 284654
• mRNA Refseq: NM_001038633.3
• Protein Refseq: NP_001033722.1
• UniProt ID: Q2MKA7