Recombinant Human RPS6KB1, GST-tagged

• Cat.No.: RPS6KB1-195H
• Product Overview: Recombinant full-length human p70S6K was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues. p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6k activity undergoes changes in the cell cycle and increases 20-fold in G1 cells released from G0. p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as threonine 389 a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK-1).
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Molecular Mass: ~76 kDa
• Purity: >90% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: RPS6KB1 ribosomal protein S6 kinase, 70kDa, polypeptide 1 [ Homo sapiens ]
• Official Symbol: RPS6KB1
• Synonyms: RPS6KB1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; ribosomal protein S6 kinase, 70kD, polypeptide 1 , STK14A; ribosomal protein S6 kinase beta-1; p70(S6K) alpha; PS6K; S6K1; P70S6K1; p70 S6KA; S6K-beta-1; p70 S6K-alpha; p70 S6 kinase, alpha 1; p70 S6 kinase, alpha 2; ribosomal protein S6 kinase I; serine/threonine kinase 14 alpha; serine/threonine-protein kinase 14A; 70 kDa ribosomal protein S6 kinase 1; S6K; STK14A; p70-S6K; p70-alpha; p70(S6K)-alpha
• Gene ID: 6198
• mRNA Refseq: NM_003161
• Protein Refseq: NP_003152
• MIM: 608938
• UniProt ID: P23443
• Chromosome Location: 17q23.1
• Pathway: Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem; Cytoplasmic Ribosomal Proteins, organism-specific biosystem; ErbB signaling pathway, organism-specific biosystem
• Function: ATP binding; nucleotide binding; peptide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; ribosomal protein S6 kinase activity