Recombinant Human Roadblock Domain Containing 3, His-tagged

• Cat.No.: ROBLD3-3389H
• Product Overview: Recombinant human ROBLD3 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.MW = 16.0 kDa (149aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: ROBLD3, also known as MAPBPIP, belongs to the GAMAD family. It is an adapter protein that enhances the efficiency of the MAP kinase cascade and facilitates the activation of MAPK2. ROBLD3 compels the recruitment of MP1 to late endosomes where they form a very stable heterodimeric complex required for ERK activation on endosomes.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M Nacl, 2mM DTT, 10% glycerol.
• Purity: > 95% by SDS - PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MGSHMLRPKA LTQVLSQANT GGVQSTLLLN NEGSLLAYSG YGDTDARVTA AIASNIWAAY DRNGNQAFNE DNLKFILMDC MEGRVAITRV ANLLLCMYAK ETVGFGMLKA KAQALVQYLE EPLTQVAAS.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: ROBLD3 roadblock domain containing 3 [ Homo sapiens ]
• Synonyms: roadblock domain containing 3;p14; ENDAP; HSPC003; MAPBPIP; MAPKSP1AP; RP11-336K24.9; ROBLD3; mitogen-activated protein-binding protein-interacting protein; MAPKSP1 adaptor protein; endosomal adaptor protein p14; roadblock domain-containing protein 3; late endosomal/lysosomal Mp1-interacting protein; mitogen-activated protein-binding protein-interacting protein (MAPBPIP); Late endosomal/lysosomal Mp1-interacting protein; Roadblock domain-containing protein; Endosomal adaptor protein p14
• Gene ID: 28956
• mRNA Refseq: NM_001145264
• Protein Refseq: NP_001138736
• MIM: 610389
• UniProt ID: Q9Y2Q5C
• Chromosome Location: 1q22