Recombinant Human RNH1 Protein, His-tagged

• Cat.No.: RNH1-2730H
• Product Overview: Recombinant Human RNH1 Protein (Ser2-Ser461) with N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ser2-Ser461
• Description: Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 53.5 kDa
  Accurate Molecular Mass: 53 kDa
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: RNH1 ribonuclease/angiogenin inhibitor 1 [ Homo sapiens (human) ]
• Official Symbol: RNH1
• Synonyms: RNH1; ribonuclease/angiogenin inhibitor 1; RAI; RNH; ribonuclease inhibitor; placental RNase inhibitor; placental ribonuclease inhibitor; testicular tissue protein Li 164
• Gene ID: 6050
• mRNA Refseq: NM_002939
• Protein Refseq: NP_002930
• MIM: 173320
• UniProt ID: P13489