Recombinant Human RNA binding motif protein 15B Protein, His&Flag&StrepII tagged

• Cat.No.: RBM15B-027H
• Product Overview: Recombinant human RBM15B protein (Q8NDT2) (Met 1-His 154) with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Flag&His&Twin Strep
• Protein Length: 1-154aa
• Description: RNA-binding protein that acts as a key regulator of N6-methyladenosine (m6A) methylation of RNAs, thereby regulating different processes, such as alternative splicing of mRNAs and X chromosome inactivation mediated by Xist RNA. Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Plays a key role in m6A methylation, possibly by binding target RNAs and recruiting the WMM complex. Involved in random X inactivation mediated by Xist RNA: acts by binding Xist RNA and recruiting the WMM complex, which mediates m6A methylation, leading to target YTHDC1 reader on Xist RNA and promoting transcription repression activity of Xist. Functions in the regulation of alternative or illicit splicing, possibly by regulating m6A methylation. Also functions as a mRNA export factor by acting as a cofactor for the nuclear export receptor NXF1.
• Tag: N-Twin strep and C-His&Flag
• Molecular Mass: 21.8 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: RBM15B RNA binding motif protein 15B [ Homo sapiens (human) ]
• Official Symbol: RBM15B
• Synonyms: RBM15B; RNA binding motif protein 15B; putative RNA-binding protein 15B; HUMAGCGB; OTT3; huOTT3; one twenty two protein 3; one-twenty two protein 3; RNA-binding motif protein 15B; chromosome 3p21.1 gene sequence
• Gene ID: 29890
• mRNA Refseq: NM_013286
• Protein Refseq: NP_037418
• MIM: 612602
• UniProt ID: Q8NDT2