Recombinant Human Ribosomal Protein S3, His-tagged

• Cat.No.: RPS3-6890H
• Product Overview: Recombinant human RPS3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 28.8 kDa.

Specification

• Description: RPS3 belongs to the S3P family of ribosomal proteins. RPS3 mediates the formation of the mRNA binding site 3" of the codon in the decoding site. In addition, RPS3 is involved in DNA damage recognition as shown by its affinity for abasic sites and 7, 8-dihydro-8-oxoguanine residues and its interaction with human base excision repair proteins OGG1 and Ref-1.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl.
• Molecular Mass: 28.8 kDa (263aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher).
• Protein length: 1-243aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAEK VATRGLCAIA QAESLRYKLL GGLAVRRACY GVLRFIMESG AKGCEVVVSG KLRGQRAKSM KFVDGLMIHS GDPVNYYVDT AVRHVLLRQG VLGIKVKIML PWDPTGKIGP KKPLPDHVSI VEPKDEILPT TPISEQKGGK PEPPAMPQPV PTA
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: RPS3 ribosomal protein S3 [ Homo sapiens ]
• Official Symbol: RPS3
• Synonyms: RPS3; ribosomal protein S3; 40S ribosomal protein S3; FLJ26283; FLJ27450; IMR 90 ribosomal protein S3; MGC87870; S3; IMR-90 ribosomal protein S3
• Gene ID: 6188
• mRNA Refseq: NM_001005
• Protein Refseq: NP_000996
• MIM: 600454
• UniProt ID: P23396
• Chromosome Location: 11q13.3-q13.5
• Pathway: Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S, organism-specific biosystem; Cap-dependent Translation Initiation, organism-specific biosystem; Cytoplasmic Ribosomal Proteins, organism-specific biosystem; Disease, organism-specific biosystem; Eukaryotic Translation Elongation, organism-specific biosystem; Eukaryotic Translation Initiation, organism-specific biosystem; Eukaryotic Translation Termination, organism-specific biosystem
• Function: DNA-(apurinic or apyrimidinic site) lyase activity; NF-kappaB binding; damaged DNA binding; endonuclease activity; iron-sulfur cluster binding; mRNA binding; protein binding; protein kinase binding; structural constituent of ribosome; structural constituent of ribosome