Recombinant Human ribosomal protein L23a, His-tagged

• Cat.No.: RPL23A-587H
• Product Overview: RPL23A, 1-156aa, Human, His tag, E.coli

Specification

• Description: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL23A is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L23P family of ribosomal proteins. It is located in the cytoplasm. The protein may be one of the target molecules involved in mediating growth inhibition by interferon. In yeast, the corresponding protein binds to a specific site on the 26S rRNA. Recombinant human RPL23A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT, 250mM imidazole
• Molecular Mass: 20.1kDa (179aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAPKAKK EAPAPPKAEA KAKALKAKKA VLKGVHSHKK KKIRTSPTFR RPKTLRLRRQ PKYPRKSAPR RNKLDHYAII KFPLTTESAM KKIEDNNTLV FIVDVKANKH QIKQAVKKLY DIDVAKVNTL IRPDGEKKAY VRLAPDYDAL DVANKIGII
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Protein length: 1-156 a.a.

Gene Information

• Gene Name: RPL23A ribosomal protein L23a [ Homo sapiens ]
• Official Symbol: RPL23A
• Synonyms: RPL23A; ribosomal protein L23a; 60S ribosomal protein L23a; L23A; melanoma differentiation-associated gene 20; MDA20; FLJ27455
• Gene ID: 6147
• mRNA Refseq: NM_000984
• Protein Refseq: NP_000975
• MIM: 602326
• UniProt ID: P62750
• Chromosome Location: 17q11.2
• Pathway: Cap-dependent Translation Initiation, organism-specific biosystem; Cytoplasmic Ribosomal Proteins, organism-specific biosystem; Disease, organism-specific biosystem; Eukaryotic Translation Elongation, organism-specific biosystem; Eukaryotic Translation Initiation, organism-specific biosystem; Eukaryotic Translation Termination, organism-specific biosystem; Formation of a pool of free 40S subunits, organism-specific biosystem
• Function: nucleotide binding; protein binding; rRNA binding; structural constituent of ribosome