Recombinant Human RFC5 protein, His & T7-tagged

• Cat.No.: RFC5-1699H
• Product Overview: Recombinant Human RFC5 aa. (Met1~Ile219 (Accession # P40937)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Met1~Ile219
• Description: This gene encodes the smallest subunit of the replication factor C complex, which consists of five distinct subunits (140, 40, 38, 37, and 36 kDa) and is required for DNA replication. This subunit interacts with the C-terminal region of proliferating cell nuclear antigen and is required to open and load proliferating cell nuclear antigen onto DNA during S phase. It is a member of the AAA+ (ATPases associated with various cellular activities) ATPase family and forms a core complex with the 38 and 40 kDa subunits that possesses DNA-dependent ATPase activity. A related pseudogene has been identified on chromosome 9. Alternative splicing results in multiple transcript variants.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 28.5kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 8.3

Gene Information

• Gene Name: RFC5 replication factor C subunit 5 [ Homo sapiens (human) ]
• Official Symbol: RFC5
• Synonyms: RFC5; replication factor C subunit 5; RFC36; replication factor C subunit 5; A1 36 kDa subunit; RF-C 36 kDa subunit; RFC, 36.5 kD subunit; replication factor C (activator 1) 5, 36.5kDa; replication factor C, 36-kDa subunit
• Gene ID: 5985
• mRNA Refseq: NM_001130112.3
• Protein Refseq: NP_001123584.1
• UniProt ID: P40937