Recombinant Human RFC2 protein, His & T7-tagged

• Cat.No.: RFC2-1703H
• Product Overview: Recombinant Human RFC2 aa. (Ser58~Asn332 (Accession # P35250)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Ser58~Asn332
• Description: This gene encodes a member of the activator 1 small subunits family. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). Replication factor C, also called activator 1, is a protein complex consisting of five distinct subunits. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP and may help promote cell survival. Disruption of this gene is associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. A pseudogene of this gene has been defined on chromosome 2.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 34.5kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 7.6

Gene Information

• Gene Name: RFC2 replication factor C subunit 2 [ Homo sapiens (human) ]
• Official Symbol: RFC2
• Synonyms: RFC2; replication factor C subunit 2; RFC40; replication factor C subunit 2; A1 40 kDa subunit; RF-C 40 kDa subunit; activator 1 subunit 2; replication factor C (activator 1) 2, 40kDa
• Gene ID: 5982
• mRNA Refseq: NM_001278791.1
• Protein Refseq: NP_001265720.1
• UniProt ID: P35250