Recombinant Human REV1, His-tagged

Description :	REV1 contains a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. Two alternatively spliced transcript variants that encode different proteins have been found. Recombinant human REV1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
Source :	E.coli
Species :	Human
Tag :	His-tag
Form :	Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
Molecular Mass :	25.2 kDa (227aa)
Protein length :	51-256aa
AA Sequence :	MGSSHHHHHH SSGLVPRGSH MGVAIYVNGY TDPSAEELRK LMMLHGGQYH VYYSRSKTTH IIATNLPNAK IKELKGEKVI RPEWIVESIK AGRLLSYIPY QLYTKQSSVQ KGLSFNPVCR PEDPLPGPSN IAKQLNNRVN HIVKKIETEN EVKVNGMNSW NEEDENNDFS FVDLEQTSPG RKQNGIPHPR GSTAIFNGHT PSSNGALKTQ DCLVPMVNSV ASRLSPA
Purity :	>85% by SDS - PAGE
Storage :	Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Concentration :	1 mg/ml (determined by Bradford assay)

Gene Name	REV1 REV1 homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol	REV1
Synonyms	REV1; REV1 homolog (S. cerevisiae); REV1 (yeast homolog) like , REV1 like (yeast) , REV1L; DNA repair protein REV1; AIBP80; REV1- like; alpha integrin-binding protein 80; rev1-like terminal deoxycytidyl transferase; REV1L; FLJ21523; MGC26225; MGC163283;
Gene ID	51455
mRNA Refseq	NM_001037872
Protein Refseq	NP_001032961
MIM	606134
UniProt ID	Q9UBZ9
Chromosome Location	2q11.1-q11.2
Pathway	DNA Damage Bypass, organism-specific biosystem; DNA Repair, organism-specific biosystem; Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem; Translesion synthesis by DNA polymerases bypassing lesion on DNA template, organism-specific biosystem; Translesion synthesis by HREV1, organism-specific biosystem;
Function	DNA-directed DNA polymerase activity; damaged DNA binding; deoxycytidyl transferase activity; magnesium ion binding; protein binding; transferase activity;