Recombinant Human RET Protein, His/GST-tagged, Alexa Fluor 555 conjugated

• Cat.No.: RET-3948HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human RET (P07949-1) cytoplasmic domain (His 658-Ser 1114), fused with the N-terminal polyhistidine-tagged GST tag, was produced in Baculovirus-Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 694
• Form: Lyophilized
• Molecular Mass: The recombinant human RET (aa 658-1114)/GST chimera consists of 694 amino acids and has a calculated molecular mass of 76.7 kDa. It migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10% gly, pH 8.0
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: RET ret proto-oncogene [ Homo sapiens ]
• Official Symbol: RET
• Gene ID: 5979
• mRNA Refseq: NM_020630
• Protein Refseq: NP_065681
• UniProt ID: P07949