Recombinant Human REST 293 Cell Lysate

• Cat.No.: REST-2417HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for RE1-silencing transcription factor (REST) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: REST RE1-silencing transcription factor [ Homo sapiens ]
• Official Symbol: REST
• Synonyms: REST; RE1-silencing transcription factor; NRSF; XBR; repressor binding to the X2 box; neural-restrictive silencer factor; neuron restrictive silencer factor
• Gene ID: 5978
• mRNA Refseq: NM_001193508
• Protein Refseq: NP_001180437
• MIM: 600571
• UniProt ID: Q13127
• Chromosome Location: 4q12
• Pathway: Huntingtons disease, organism-specific biosystem; Huntingtons disease, conserved biosystem; SIDS Susceptibility Pathways, organism-specific biosystem
• Function: DNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; calcium channel activity; chromatin binding; core promoter binding; core promoter sequence-specific DNA binding; metal ion binding; outward rectifier potassium channel activity; protein binding; protein complex binding; sequence-specific DNA binding transcription factor activity; transcription factor binding; transcription regulatory region DNA binding; zinc ion binding