Recombinant Human resistin like beta, His-tagged

• Cat.No.: RETNLB-123H
• Product Overview: Recombinant Human RELM-beta is a His-Tagged Fusion Protein which is 11 kDa protein containing 90 amino acid residues of the RELM-beta human and 12 additional amino acid residues –His Tag (underlined).

Specification

• Cat. No.: RETNLB-123H
• Description: RELM-beta (Resistin-Like Molecule-beta) is a member of a recently identified family of secreted proteins containing a conserved cystein-rich C-terminus. RELM-beta appears to be produced as a homodimer exclusively by intestinal goblet cells and can be found in high quantities in stool. Remarkably, stool of germ-free mice displaying sterile intestinal tract does not contain RELM-beta until bacterial colonization takes place after pathogen-free mice entered natural environment. High doses of recombinant RELM-beta showed hyperglycemic effects including lowered glucose disposal and increased hepatic glucose production in mice.
• Source: Escherichia Coli.
• Amino acid sequence: MGSTQCSLDS VMDKKIKDVL NSLEYSPSPI SKKLSCASVK SQGRPSSCPA GMAVTGCACG YGCGSWDVQLETTCHCQCSV VDWTTARCCH LTKLRSHHHH HH.
• Specificity: The amino acid sequence of the RELM-beta human recombinant is 100% homologous to the amino acid sequence of the RELM-beta human without signal sequence.
• Purification Method: Three-step procedure using affinity Ni-NTA chromatography and size exclusionchromatography before and after refolding.
• Purity: >95% (SDS-PAGE analyzed).
• Formulation: Sterile filtered and lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH-4.
• Reconstitution: Add 0.2 ml of 0.1 M Acetate buffer pH-4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited.
• Stability/Shelf Life: The lyophilized protein remains stable until the expiry date when stored at -20°C.
• Applications: Western blotting.
• Storage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Gene Information

• Gene Name: RETNLB resistin like beta [ Homo sapiens ]
• Synonyms: RETNLB; resistin like beta; XCP2; FIZZ1; FIZZ2; HXCP2; RELMb; RELMbeta; RELM-beta; found in inflammatory zone 1; cysteine-rich secreted A12-alpha-like protein 1; colon and small intestine-specific cysteine-rich protein; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 2; CCRG; RETNL2
• Gene ID: 84666
• mRNA Refseq: NM_032579
• Protein Refseq: NP_115968
• MIM: 605645
• UniProt ID: Q9BQ08
• Chromosome Location: 3q13.1
• Function: hormone activity; molecular_function