Recombinant Human REL 293 Cell Lysate

• Cat.No.: REL-2424HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for v-rel reticuloendotheliosis viral oncogene homolog (avian) (REL) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: REL v-rel reticuloendotheliosis viral oncogene homolog (avian) [ Homo sapiens ]
• Official Symbol: REL
• Synonyms: REL; v-rel reticuloendotheliosis viral oncogene homolog (avian); v rel avian reticuloendotheliosis viral oncogene homolog; proto-oncogene c-Rel; c Rel; I Rel; C-Rel proto-oncogene protein; oncogene REL, avian reticuloendotheliosis; v-rel avian reticuloendotheliosis viral oncogene homolog; C-Rel
• Gene ID: 5966
• mRNA Refseq: NM_002908
• Protein Refseq: NP_002899
• MIM: 164910
• UniProt ID: Q04864
• Chromosome Location: 2p13-p12
• Pathway: Activation of NF-kappaB in B Cells, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Atypical NF-kappaB pathway, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Downstream Signaling Events Of B Cell Receptor (BCR), organism-specific biosystem; Immune System, organism-specific biosystem; Regulation of Androgen receptor activity, organism-specific biosystem
• Function: DNA binding; protein binding; sequence-specific DNA binding transcription factor activity