Recombinant Human regulatory factor X-associated ankyrin-containing protein, His-tagged

• Cat.No.: RFXANK-234H
• Product Overview: RFXANK, 1-237aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-237 a.a.
• Description: RFXANK, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Two transcript variants encoding different isoforms have been described for this gene, with only one isoform showing activation activity. Recombinant human RFXANK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol.
• Molecular Mass: 28 kDa (260aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMELTQPA EDLIQTQQTP ASELGDPEDP GEEAADGSDT VVLSLFPCTP EPVNPEPDAS VSSPQGSSLK HSTTLTNRQR GNEVSALPAT LDCDNLVNKP DERGFTPLIW ASAFGEIETV RFLLEWGADP HILAKERESA LSLASTGGYT DIVGLLLERD VDINIYDWNG GTPLLYAVRG NHVKCVEALL ARGADLTTEA DSGYTPMDLA VALGYRKVQQ VIENHILKLF QSNLVPADPE
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: RFXANK regulatory factor X-associated ankyrin-containing protein [ Homo sapiens ]
• Official Symbol: RFXANK
• Synonyms: RFXANK; regulatory factor X-associated ankyrin-containing protein; DNA-binding protein RFXANK; ANKRA1; ankyrin repeat containing regulatory factor X associated protein; BLS; DNA binding protein RFXANK; F14150_1; MGC138628; regulatory factor X subunit B; RFX B; RFX Bdelta4; RFX-Bdelta4; ankyrin repeat family A protein 1; ankyrin repeat-containing regulatory factor X-associated protein; RFX-B
• Gene ID: 8625
• mRNA Refseq: NM_003721
• Protein Refseq: NP_003712
• MIM: 603200
• UniProt ID: O14593
• Chromosome Location: 19p12
• Pathway: Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; EGFR1 Signaling Pathway, organism-specific biosystem; Primary immunodeficiency, organism-specific biosystem; Primary immunodeficiency, conserved biosystem; Signaling events mediated by HDAC Class II, organism-specific biosystem; Tuberculosis, organism-specific biosystem
• Function: DNA binding; histone deacetylase binding; sequence-specific DNA binding transcription factor activity; transcription cofactor activity