Recombinant Human Regenerating Islet-derived 1 Beta, His-tagged

• Cat.No.: REG1B-193H
• Product Overview: Recombinant Human REG 1 beta (His-tagged) produced inE.colicontains 144 amino acids and has a molecular mass of 17.8 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Reg protein was shown to be stimulated during the regeneration of pancreatic islets. Since then, many Reg-related proteins have been identified in humans and other animals. In human, the four REG family genes, i.e., REG 1 alpha, REG 1 beta, REG-related sequence (RS) and HIP/PAP, have so far been isolated. These Reg-related proteins are classified into four subfamilies according to their amino-acid sequences, but they share a similar structure and physiological function. Reg protein is a growth factor for pancreatic beta cells and also suggest that the administration of Reg protein could be used as another therapeutic approach for diabetes mellitus. human REG cDNA which encoded a 166-amino acid protein with a 22-amino acid signal peptide. The amino acid sequence of human REG protein has 68% homology to that of rat Reg protein. Reg I was found to be expressed mainly in pancreatic beta and acinoductular cells as well as gastric fundic enterochromaffin-like (ECL) cells. Reg I production in ECL cells is stimulated by gastrin, as well as by the proinflammatory cytokine, cytokine-induced neutrophil chemoattractant (CINC)-2Beta.
• Sequences Of Amino Acids: MKHHHHHHAS HMQESQTELP NPRISCPEGT NAYRSYCYYF NEDPETWVDA DLYCQNMNSG NLVSVLTQAE GAFVASLIKE SSTDDSNVWI GLHDPKKNRR WHWSSGSLVS YKSWDTGSPS SANAGYCASL TSCSGFKKWK DESCEKKFSF VCKFKN.
• Specificity: The amino acid sequence of the recombinant Human REG 1 beta is 100% homologous to the amino acid sequence of the Human REG 1 beta without signal sequence.
• Purification Method: Two-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography.
• Purity: Purity of recombinant Human REG 1 beta is >95% (SDS-PAGE analyzed).
• Formulation: Sterile filtered and lyophilized from 0.5 mg/ml in 20 mM Tris , pH 8.0.
• Reconstitution: Add 0.2 ml of deionized H₂O and let the lyophilized pellet dissolve completely.
• Stability/Shelf Life: The lyophilized protein remains stable until the expiry date when stored at -20°C.
• Applications: Western blotting, ELISA.
• Storage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Gene Information

• Gene Name: REG1B regenerating islet-derived 1 beta [ Homo sapiens ]
• Synonyms: REG1B; PSPS2; REGH; REGI-BETA; REGL; Regenerating protein I beta; lithostathine 1 beta; regenerating islet-derived 1 beta; secretory pancreatic stone protein; regenerating islet-derived 1 beta (pancreatic stone protein, pancreatic thread protein); Regenerating islet-derived 1 beta (pancreatic stone protein, pancreatic thread protein); secretory pancreatic stone protein 2
• Gene ID: 5968
• mRNA Refseq: NM_006507
• Protein Refseq: NP_006498
• MIM: 167771
• UniProt ID: P48304
• Chromosome Location: 2p12
• Function: binding; sugar binding