Recombinant Human REG4, His-tagged

• Cat.No.: REG4-194H
• Product Overview: Recombinant Human regenerating islet-derived family, member 4 (His-tagged) contains 136 amino acids and has a molecular mass of 17.4 kDa. And this protein expressed inE.Coli.

Specification

• Cat. No.: REG4-194H
• Description: REG protein was shown to be stimulated during the regeneration of pancreatic islets. Since then, many Reg-related proteins have been identified in humans and other animals. In human, the four REG family genes, i.e., REG 1 alpha, REG 1 beta, REG-related sequence (RS) and HIP/PAP, have so far been isolated. These Reg-related proteins are classified into four subfamilies according to their amino-acid sequences, but they share a similar structure and physiological function. Reg protein is a growth factor for pancreatic beta cells and also suggest that the administration of Reg protein could be used as another therapeutic approach for diabetes mellitus. human REG cDNA which encoded a 166-amino acid protein with a 22-amino acid signal peptide. The amino acid sequence of human REG protein has 68% homology to that of rat Reg protein. Reg I was found to be expressed mainly in pancreatic beta and acinoductular cells as well as gastric fundic enterochromaffin-like (ECL) cells. Reg I production in ECL cells is stimulated by gastrin, as well as by the proinflammatory cytokine, cytokine-induced neutrophil chemoattractant (CINC)-2Beta.
• Source: E.Coli.
• Sequences Of Amino Acids: MKHHHHHHAS HMDIIMRPSC APGWFYHKSN CYGYFRKLRN WSDAELECQS YGNGAHLASI LSLKEASTIA EYISGYQRSQ PIWIGLHDPQ KRQQWQWIDG AMYLYRSWSG KSMGGNKHC AEMSSNNNFLT WSSNECNKRQ HFLCKYRP.
• Specificity: The amino acid sequence of the recombinant Human REG IV is 100% homologous to the amino acid sequence of the Human REG IV without signal sequence.
• Purification Method: Ni-NTA chromatography.
• Purity: >95% (SDS-PAGE analyzed).
• Formulation: Sterile filtered and lyophilized from 0.5 mg/ml in 20 mM Tris , pH 8.0.
• Reconstitution: Add 0.2 ml of deionized H₂O and let the lyophilized pellet dissolve completely.
• Stability/Shelf Life: The lyophilized protein remains stable until the expiry date when stored at -20°C.
• Applications: Western blotting, ELISA.
• Storage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Gene Information

• Gene Name: REG4 regenerating islet-derived family, member 4 [ Homo sapiens ]
• Synonyms: REG4; GISP; REG-IV; RELP; OTTHUMP00000014030; OTTHUMP00000014031; Reg IV; REG-like protein; regenerating gene type IV; Gastrointestinal secretory protein; regenerating islet-derived family, member 4; Regenerating islet-derived family, member 4
• Gene ID: 83998
• mRNA Refseq: NM_001159352
• Protein Refseq: NP_001152824
• MIM: 609846
• UniProt ID: Q9BYZ8
• Chromosome Location: 1p13.1-p12
• Function: binding; sugar binding