Recombinant Human RDH12, His-tagged

• Cat.No.: RDH12-31307TH
• Product Overview: Recombinant fragment of Human RDH12 (amino acids 39-316) fused to His tag at N-terminus; 303aa, 33.5kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 278 amino acids
• Description: The protein encoded by this gene is an NADPH-dependent retinal reductase whose highest activity is toward 9-cis and all-trans-retinol. The encoded enzyme also plays a role in the metabolism of short-chain aldehydes but does not exhibit steroid dehydrogenase activity. Defects in this gene are a cause of Leber congenital amaurosis type 3 (LCA3).
• Conjugation: HIS
• Molecular Weight: 33.500kDa inclusive of tags
• Tissue specificity: Widely expressed, mostly in eye, kidney, brain, skeletal msucle and stomach.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.32% Tris HCl, 1.17% Sodium chloride, 0.03% DTT, 40% Glycerol
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMSIRAFAEGFL AEEKQLHILI NNAGVMMCPY SKTADGFETH LGVNHLGHFL LTYLLLERLK VSAPARVVNV SSVAHHIGKI PFHDLQSEKR YSRGFAYCHS KLANVLFTRE LAKRLQGTGV TTYAVHPGVV RSELVRHSSL LCLLWRLFSP FVKTAREGAQ TSLHCALAEG LEPLSGKYFS GKVVV ITGANTGIGK ETARELASRG ARVYIACRDV LKGESAASEI RVDTKNSQVL VRKLDLSDTKDCKRTWVSPR ARNNKTAERL WNVSCELLGI RWE
• Sequence Similarities: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Gene Information

• Gene Name: RDH12 retinol dehydrogenase 12 (all-trans/9-cis/11-cis) [ Homo sapiens ]
• Official Symbol: RDH12
• Synonyms: RDH12; retinol dehydrogenase 12 (all-trans/9-cis/11-cis); retinol dehydrogenase 12 (all trans and 9 cis); retinol dehydrogenase 12; FLJ30273; LCA13; SDR7C2; short chain dehydrogenase/reductase family 7C; member 2
• Gene ID: 145226
• mRNA Refseq: NM_152443
• Protein Refseq: NP_689656
• MIM: 608830
• Uniprot ID: Q96NR8
• Chromosome Location: 14q24.1
• Pathway: Metabolic pathways, organism-specific biosystem; Retinol metabolism, organism-specific biosystem; Retinol metabolism, conserved biosystem; Visual signal transduction: Cones, organism-specific biosystem; Visual signal transduction: Rods, organism-specific biosystem
• Function: nucleotide binding; oxidoreductase activity; retinol dehydrogenase activity