Recombinant Human RBP4 protein, His-tagged

• Cat.No.: RBP4-442H
• Product Overview: Recombinant Human RBP4 (P02753) aa. (Ala18~Leu201) fused with His tag was produced in HEK293F cells.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Ala18~Leu201
• Description: This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 22.7kDa; Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 95%
• Characteristic: Isoelectric point is 5.3.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: RBP4 retinol binding protein 4 [ Homo sapiens (human) ]
• Official Symbol: RBP4
• Synonyms: RBP4; retinol binding protein 4; RDCCAS; MCOPCB10; PRBP; RBP; plasma retinol-binding protein; retinol binding protein 4, plasma; retinol-binding protein 4, interstitial
• Gene ID: 5950
• mRNA Refseq: NM_001323517.1
• Protein Refseq: NP_001310446.1
• UniProt ID: P02753