Name: Recombinant Human RASAL2 293 Cell Lysate
SKU: RASAL2-2509HCL

Species :	Human
Source :	HEK293
Tag :	Non
Description :	Antigen standard for RAS protein activator like 2 (RASAL2), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Name	RASAL2 RAS protein activator like 2 [ Homo sapiens ]
Official Symbol	RASAL2
Synonyms	NGAP
Gene ID	9462
mRNA Refseq	NM_170692.2
Protein Refseq	NP_733793.2
MIM	606136
UniProt ID	Q9UJF2
Chromosome Location	1q24
Pathway	TNF-alpha/NF-kB Signaling Pathway, organism-specific biosystem;
Function	Ras GTPase activator activity;phospholipid binding;