Recombinant Human ras homolog family member Q, His-tagged

• Cat.No.: RHOQ-566H
• Product Overview: RHOQ, 1-202aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-202 a.a.
• Description: RHOQ, also known as rho-related GTP-binding protein RhoQ, is a small signaling G protein (more specifically a GTPase), and is a member of the Rac subfamily of the family Rho family of GTPases. It involved in epithelial cell polarization processes. It may play a role in CFTR trafficking to the plasma membrane. It causes the formation of thin, actin-rich surface projections called filopodia. Recombinant human RhoQ protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT
• Molecular Mass: 24.7kDa (225aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAHGPGA LMLKCVVVGD GAVGKTCLLM SYANDAFPEE YVPTVFDHYA VSVTVGGKQY LLGLYDTAGQ EDYDRLRPLS YPMTDVFLIC FSVVNPASFQ NVKEEWVPEL KEYAPNVPFL LIGTQIDLRD DPKTLARLND MKEKPICVEQ GQKLAKEIGA CCYVECSALT QKGLKTVFDE AIIAILTPKK HTVKKRIGSR CINCC
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: RHOQ ras homolog family member Q [ Homo sapiens ]
• Official Symbol: RHOQ
• Synonyms: RHOQ; ras homolog family member Q; ARHQ, ras homolog gene family, member Q , RAS like, family 7, member A , RASL7A; rho-related GTP-binding protein RhoQ; TC10; ras-like protein TC10; RAS-like, family 7, member A; ras homolog gene family, member Q; ras-like protein family member 7A; ARHQ; TC10A; RASL7A
• Gene ID: 23433
• mRNA Refseq: NM_012249
• Protein Refseq: NP_036381
• MIM: 605857
• UniProt ID: P17081
• Chromosome Location: 2p21
• Pathway: Insulin Pathway, organism-specific biosystem; Insulin Signaling, organism-specific biosystem; Insulin signaling pathway, organism-specific biosystem; Insulin signaling pathway, conserved biosystem; Insulin-mediated glucose transport, organism-specific biosystem; Rho GTPase cycle, organism-specific biosystem; Signal Transduction, organism-specific biosystem
• Function: GBD domain binding; GTP binding; GTPase activity; nucleotide binding; profilin binding