Recombinant Human RAP1B, His-tagged

• Cat.No.: RAP1B-3469H
• Product Overview: Recombinant human RAP1B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: RAP1B belongs to a superfamily of RAS-like small GTP-binding proteins involved in cell signaling. It possesses intrinsic GTPase activity and is ubiquitously expressed in mammalian tissues. The putative effector domain of Ras proteins, whose integrity is required for cell transformation as well as interaction with the putative effector protein GAP, is conserved in both Rap 1 proteins.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol
• Molecular Weight: 22.6 kDa (201aa), confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDAQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTDDVP MILVGNKCDL EDERVVGKEQ GQNLARQWNN CAFLESSAKS KINVNEIFYD LVRQINRKTP VPGKARKKSS C
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: RAP1B RAP1B, member of RAS oncogene family [ Homo sapiens ]
• Synonyms: RAP1B, member of RAS oncogene family; K-REV; RAL1B; DKFZp586H0723; RAP1B; ras-related protein Rap-1b; OTTHUMP00000165028; RAS-related protein RAP1B; small GTP binding protein; GTP-binding protein smg p21B; Ras family small GTP binding protein RAP1B; OK/SW-cl.11
• Gene ID: 5908
• mRNA Refseq: NM_001010942
• Protein Refseq: NP_001010942
• MIM: 179530
• UniProt ID: P61224
• Chromosome Location: 12q14
• Pathway: Renal cell carcinoma; Pancreatic secretion; Neurotrophin signaling pathway; Long-term potentiation; Leukocyte transendothelial migration; Focal adhesion; Chemokine signaling pathway; MAPK signaling pathway; Hemostasis; Diabetes pathways; Integration of energy metabolism; Signaling in Immune system; ntegrin cell surface interactions
• Function: GDP binding; GTP binding; GTPase activity; nucleotide binding; protein binding