Recombinant Human RAF1 Protein, His-tagged
Cat.No. : | RAF1-19H |
Product Overview : | Human Ras Binding Domain (RBD) of Raf-1 Proto-Oncogene, Serine/Threonine Kinase (RAF1), (UniProt # P04049, amino acids 1-149), expressed with an N-terminal 6XHis and glutathione S-transferase (GST) dual tag in E. coli. The dual 6xHis-GST tag allows immobilization of Raf1 RBD Protein and RBD-Ras complexes on either Ni-NTA or glutathione agarose resin columns. |
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Species : | Human |
Source : | E.coli |
Tag : | His |
Protein Length : | 1-149 a.a. |
Description : | The Raf1 RBD protein binds specifically to GTP-bound Ras proteins. Ras proteins, like other small GTPases, regulate molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Ras binds specifically to the Ras-binding domain (RBD) of Raf1 to control downstream signaling cascades. The affinity between RBD and GDP-bound Ras is three orders of magnitude lower. Therefore, the RBD protein can be used to specifically precipitate active, GTP-bound Ras as well as to specifically block the activity of Ras in vitro and in vivo. |
Purity : | > 80% by Coomassie staining |
Applications : | Measurement of the GTP/GDP bound ratio of Ras in vitro. Quantitation of GTP-bound Ras from tissue and tissue culture cell lysates. RAS/RAF binding studies. Inhibitor screening for RAS/RAF interaction |
Notes : | This product is for laboratory research use only. |
Storage : | Storage is recommended at -20 centigrade for longer periods of time. |
Concentration : | 1.0 mg/mL by A280 |
Storage Buffer : | 20 mM HEPES, pH 7.5, 100 mM NaCl, and 20% Glycerol. |
Shipping : | Product requires shipping on ice packs. |
Gene Name | RAF1 Raf-1 proto-oncogene, serine/threonine kinase [ Homo sapiens (human) ] |
Official Symbol | RAF1 |
Synonyms | RAF1; Raf-1 proto-oncogene, serine/threonine kinase; NS5; CRAF; Raf-1; c-Raf; CMD1NN; RAF proto-oncogene serine/threonine-protein kinase; C-Raf proto-oncogene, serine/threonine kinase; Oncogene RAF1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1; EC 2.7.11.1 |
Gene ID | 5894 |
mRNA Refseq | NM_002880 |
Protein Refseq | NP_002871 |
MIM | 164760 |
UniProt ID | P04049 |
◆ Recombinant Proteins | ||
RAF1-424HF | Recombinant Full Length Human RAF1 Protein, GST-tagged | +Inquiry |
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RAF1-416H | Active Recombinant Human RAF1 protein Y340E Y341E, GST-tagged | +Inquiry |
RAF1-1122H | Recombinant Human V-raf-1 Murine Leukemia Viral Oncogene Homolog 1 | +Inquiry |
◆ Cell & Tissue Lysates | ||
RAF1-1463HCL | Recombinant Human RAF1 cell lysate | +Inquiry |
Not For Human Consumption!
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