Recombinant Human RAF1, MYC/DDK-tagged

• Cat.No.: RAF1-552H
• Product Overview: Recombinant Human RAF1, fused with C-terminal MYC/DDK, was expressed in HEK293 cells.

Specification

• Description: This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
• Source: HEK293 cells
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 72.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: RAF1 Raf-1 proto-oncogene, serine/threonine kinase [ Homo sapiens (human) ]
• Official Symbol: RAF1
• Synonyms: RAF1; v-raf-1 murine leukemia viral oncogene homolog 1; NS5; CRAF; Raf-1; c-Raf; Oncogene RAF1; raf proto-oncogene serine/threonine protein kinase; EC 2.7.11.1; C-RAF; cRaf
• Gene ID: 5894
• mRNA Refseq: NM_002880
• Protein Refseq: NP_002871
• MIM: 164760
• UniProt ID: P04049
• Chromosome Location: 3p25
• Pathway: Activation of NMDA receptor upon glutamate binding and postsynaptic events; Acute myeloid leukemia; Axon guidance; B cell receptor signaling pathway
• Function: ATP binding; MAP kinase kinase kinase activity; identical protein binding; mitogen-activated protein kinase kinase binding