Recombinant Human RAD51 Protein (G151D mutated), Sumo/His/Strep tagged

• Cat.No.: RAD51-03H
• Product Overview: Recombinant Human RAD51 protein (G151D mutated) with Sumo/His/Strep tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His&Strep&SUMO
• Molecular Mass: The protein has a calculated MW of 51.11 kDa.
• AA Sequence: MNWSHPQFEKSSGSSGGHHHHHHGGSGGSGSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGGMAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILTESRSVARLECNSVILVYCTLRLSGSSDSPASASRVVGTTGGIETGSITEMFGEFRTGKTQICHTLAVTCQLPIDRDGGEGKAMYIDTEGTFRPERLLAVAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKDC
• Purity: > 85% by SDS-PAGE
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.4 mg/mL
• Storage Buffer: 20 mM Tris-HCl, 100 mM KCl, 0.5mM EDTA, 10% Glycerol, pH 8.0

Gene Information

• Gene Name: RAD51 RAD51 homolog (S. cerevisiae) [ Homo sapiens (human) ]
• Official Symbol: RAD51
• Synonyms: RAD51; RAD51 homolog (S. cerevisiae); RAD51 (S. cerevisiae) homolog (E coli RecA homolog), RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae), RAD51A, RECA; DNA repair protein RAD51 homolog 1; BRCA1/BRCA2 containing complex; subunit 5; BRCC5; HsRad51; HsT16930; RAD51 homolog A; RecA-like protein; recombination protein A; RecA, E. coli, homolog of; RAD51 homolog (RecA homolog, E. coli); BRCA1/BRCA2-containing complex, subunit 5; RECA; MRMV2; HRAD51; RAD51A
• Gene ID: 5888
• mRNA Refseq: NM_001164269
• Protein Refseq: NP_001157741
• MIM: 179617
• UniProt ID: Q06609