Recombinant Human RAD51 (G151D) Mutation Protein, Trx/His-tagged

• Cat.No.: RAD51-01H
• Product Overview: Recombinant Human RAD51 (G151D) Mutation Protein with a Trx/His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene.
• Molecular Mass: 54 kDa
• AA Sequence: MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLAGSGSGHMHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKMAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILTESRSVARLECNSVILVYCTLRLSGSSDSPASASRVVGTTGGIETGSITEMFGEFRTGKTQICHTLAVTCQLPIDRDGGEGKAMYIDTEGTFRPERLLAVAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.2 mg/mL
• Storage Buffer: PBS buffer
• Publications:
  Efficient Zygotic Genome Editing via RAD51-Enhanced Interhomolog Repair (2018)
  Efficient embryonic homozygous gene conversion via RAD51-enhanced interhomolog repair (2021)

Gene Information

• Gene Name: RAD51 RAD51 recombinase [ Homo sapiens (human) ]
• Official Symbol: RAD51
• Synonyms: RAD51; RAD51 recombinase; RECA; BRCC5; FANCR; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930; DNA repair protein RAD51 homolog 1; BRCA1/BRCA2-containing complex, subunit 5; RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A
• Gene ID: 5888
• mRNA Refseq: NM_133487
• Protein Refseq: NP_597994
• MIM: 179617
• UniProt ID: Q06609