Recombinant Human RACGAP1 protein, His & GST-tagged

• Cat.No.: RACGAP1-1191H
• Product Overview: Recombinant Human RACGAP1 aa. (Met106~Trp539 (Accession # Q9H0H5) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&GST
• ProteinLength: Met106~Trp539
• Description: This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 78.1kDa.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 8.9.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: RACGAP1 Rac GTPase activating protein 1 [ Homo sapiens (human) ]
• Official Symbol: RACGAP1
• Synonyms: RACGAP1; Rac GTPase activating protein 1; CYK4; ID-GAP; HsCYK-4; MgcRacGAP; rac GTPase-activating protein 1; male germ cell RacGap; protein CYK4 homolog
• Gene ID: 29127
• mRNA Refseq: NM_001126103.2
• Protein Refseq: NP_001119575.1
• UniProt ID: Q9H0H5