Recombinant Human RAB35, Member RAS Oncogene Family, His-tagged

• Cat.No.: RAB35-6869H
• Product Overview: Recombinant human RAB35 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 25.2 kDa.

Specification

• Description: RAB35 is localized to the plasma membrane and endocytic compartments and controls a fast endocytic recycling pathway. Consistent with a key requirement for Rab35-regulated recycling during cell division, inhibition of Rab35 function leads to the accumulation of endocytic markers on numerous cytoplasmic vacuoles in cells that failed cytokinesis.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT.
• Molecular Mass: 25.2 kDa (221aa), confirmed by MALDI-TOF.
• Protein length: 1-203aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MARDYDHLFK LLIIGDSGVG KSSLLLRFAD NTFSGSYITT IGVDFKIRTV EINGEKVKLQ IWDTAGQERF RTITSTYYRG THGVIVVYDV TSAESFVNVK RWLHEINQNC DDVCRILVGN KNDDPERKVV ETEDAYKFAG QMGIQLFETS AKENVNVEEM FNCITELVLR AKKDNLAKQQ QQQQNDVVKL TKNSKRKKRC C
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: RAB35 RAB35, member RAS oncogene family [ Homo sapiens ]
• Official Symbol: RAB35
• Synonyms: RAB35; RAB35, member RAS oncogene family; ras-related protein Rab-35; H ray; GTP-binding protein RAY; ras-related protein rab-1c (GTP-binding protein ray); RAY; H-ray; RAB1C
• Gene ID: 11021
• mRNA Refseq: NM_001167606
• Protein Refseq: NP_001161078
• MIM: 604199
• UniProt ID: Q15286
• Chromosome Location: 12q24
• Function: GTP binding; GTPase activity; nucleotide binding; phosphatidylinositol-4,5-bisphosphate binding