Recombinant Human RAB32, Member RAS Oncogene Family, His-tagged

• Cat.No.: RAB32-2495H
• Product Overview: Recombinant human RAB32 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 27.6 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-225aa
• Description: RAB32 belongs to the small GTPase superfamily. RAB32 modulates ER calcium handling and disrupts the specific enrichment of calnexin on the MAM (mitochondria-associated membrane), while not affecting the ER distribution of protein-disulfide isomerase and mitofusin-2. Also, RAB32 determines the targeting of PKA (cAMP-dependent protein kinase) to mitochondrial and ER membranes and through its overexpression or inactivation increases the phosphorylation of Bad and of Drp1.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 50% glycerol, 200mM NaCl, 2mM EDTA.
• Molecular Mass: 27.6kDa (249aa), confirmed by MALDI-TOF.
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAGGGA GDPGLGAAAA PAPETREHLF KVLVIGELGV GKTSIIKRYV HQLFSQHYRA TIGVDFALKV LNWDSRTLVR LQLWDIAGQE RFGNMTRVYY KEAVGAFVVF DISRSSTFEA VLKWKSDLDS KVHLPNGSPI PAVLLANKCD QNKDSSQSPS QVDQFCKEHG FAGWFETSAK DNINIEEAAR FLVEKILVNH QSFPNEENDV DKIKLDQETL RAENKSQCC
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: RAB32 RAB32, member RAS oncogene family [ Homo sapiens ]
• Official Symbol: RAB32
• Synonyms: RAB32; RAB32, member RAS oncogene family; ras-related protein Rab-32
• Gene ID: 10981
• mRNA Refseq: NM_006834
• Protein Refseq: NP_006825
• MIM: 612906
• UniProt ID: Q13637
• Chromosome Location: 6q24.2
• Function: GTP binding; nucleotide binding