Recombinant Human PYGB Protein, N-His-tagged

• Cat.No.: PYGB-12H
• Product Overview: Recombinant Human PYGB Protein, fused to His at N-terminus, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Val650 - Asp843
• Description: The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation.
• Form: Lyophilized from a 0.2 μm filtered 50 mM Tris-HCl (pH 7.4) solution with the protein concentration of 1.2 mg/mL.
• Molecular Mass: 22 kDa
• Endotoxin: Not determined
• Purity: > 95%
• Applications: ELISA, Western blotting, Dot Blotting, Immunogen, Protein Array
• Storage: -20 centigrade
• Shipping: Ice packs
• Use/Stability: Upon arrival, the lyophilized protein may be stored for 2 weeks at 4 centigrade. For long term storage, it is recommended to store desiccated below -20 centigrade in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 centigrade. For long term storage, it is recommended to make appropriate aliquots and store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Reconstitution: Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.1~ 1 mg/mL with sterile ice-cold deionized water or 1X Phosphate Buffered Saline (PBS). This solution can then be further diluted into other aqueous buffers.

Gene Information

• Gene Name: PYGB glycogen phosphorylase B [ Homo sapiens (human) ]
• Official Symbol: PYGB
• Synonyms: PYGB; glycogen phosphorylase B; GPBB; Glycogen phosphorylase; brain form
• Gene ID: 5834
• mRNA Refseq: NM_002862.4
• Protein Refseq: NP_002853.2
• MIM: 138550
• UniProt ID: P11216