Recombinant Human PUF60 protein, His & GST-tagged

• Cat.No.: PUF60-8041H
• Product Overview: Recombinant Human PUF60 aa. (Asn226~Gln549 (Accession # Q9UHX1)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Asn226~Gln549
• Description: This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 64.6kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 5.0.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: PUF60 poly(U) binding splicing factor 60 [ Homo sapiens (human) ]
• Official Symbol: PUF60
• Synonyms: FIR; VRJS; RoBPI; SIAHBP1; FBP interacting repressor; FUSE-binding protein-interacting repressor; Ro ribonucleoprotein-binding protein 1; Siah binding protein 1; poly(U) binding splicing factor 60KDa; pyrimidine tract binding splicing factor; poly(U)-binding-splicing factor PUF60
• Gene ID: 22827
• mRNA Refseq: NM_001136033.2
• Protein Refseq: NP_001129505.1
• UniProt ID: Q9UHX1