Recombinant Human PTGS2 Protein, His-tagged

• Cat.No.: PTGS2-2640H
• Product Overview: Recombinant Human PTGS2 Protein (Asn19-Asp589) with a N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Asn19-Asp589
• Description: Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 69.1 kDa
  Accurate Molecular Mass: 67 kDa
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PTGS2 prostaglandin-endoperoxide synthase 2 [ Homo sapiens (human) ]
• Official Symbol: PTGS2
• Synonyms: PTGS2; prostaglandin-endoperoxide synthase 2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS; prostaglandin G/H synthase 2
  PGH synthase 2
  PHS II
  cyclooxygenase 2
  cyclooxygenase 2b
  prostaglandin H2 synthase 2
  prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
  EC 1.14.99.1
• Gene ID: 5743
• mRNA Refseq: NM_000963
• Protein Refseq: NP_000954
• MIM: 600262
• UniProt ID: P35354