Recombinant Human PTEN Protein, His-tagged

• Cat.No.: PTEN-078H
• Product Overview: Human recombinant N-terminal His-tagged PPP2CA protein purified from Sf21 cells.

Specification

• Description: This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
• Source: Insect cell
• Species: Human
• Tag: His
• Molecular Mass: 50.8 kDa
• Purity: ≥95% as determined by SDS-PAGE
• Stability: ≥ 6 months
• Storage: At -80 centigrade.
• Storage Buffer: 50 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 5 mM DTT, and 20% glycerol

Gene Information

• Gene Name: PTEN phosphatase and tensin homolog [ Homo sapiens (human) ]
• Official Symbol: PTEN
• Synonyms: PTEN; phosphatase and tensin homolog; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; PTENepsilon; mitochondrial PTENalpha; mitochondrial phosphatase and tensin protein alpha; mutated in multiple advanced cancers 1; phosphatase and tensin-like protein; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; protein tyrosine phosphatase; EC 3.1.3.16; EC 3.1.3.48; EC 3.1.3.67
• Gene ID: 5728
• mRNA Refseq: NM_000314
• Protein Refseq: NP_000305
• MIM: 601728
• UniProt ID: P60484