Recombinant Human PSMB5, 60-263 aa, His-tagged

• Cat.No.: PSMB5-3374H
• Product Overview: Recombinant human PSMB5 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.MW = 26.7 kDa (241aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: PSMB5 is a member of the proteasome B-type family that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). PSMB5 has an ATP dependent proteolytic activity and involved in an ATP/ubiquitin dependent non lysosomal proteolytic pathway.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 20% glycerol 5mM DTT, 0.2M NaCl.
• Purity: > 90% by SDS - PAGE.
• Concentration: 0.25 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMTTT LAFKFRHGVI VAADSRATAG AYIASQTVKK VIEINPYLLG TMAGGAADCS FWERLLARQC RIYELRNKER ISVAAASKLL ANMVYQYKGM GLSMGTMICG WDKRGPGLYY VDSEGNRISG ATFSVGSGSV YAYGVMDRGY SYDLEVEQAY DLARRAIYQA TYRDAYSGGA VNLYHVREDG WIRVSSDNVA DLHEKYSGST P.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Proteasome; Apoptosis; Cell Cycle Checkpoints; Cell Cycle, Mitotic; DNA Replication; HIV Infection; Metabolism of amino acids and derivatives; Signaling by Wnt

Gene Information

• Gene Name: PSMB5 proteasome (prosome, macropain) subunit, beta type, 5 [ Homo sapiens ]
• Synonyms: proteasome (prosome, macropain) subunit, beta type, 5; X; MB1; LMPX; MGC104214; PSMB5; proteasome subunit beta type-5; proteasome chain 6; proteasome subunit X; proteasome subunit MB1; macropain epsilon chain; proteasome epsilon chain; proteasome beta 5 subunit; proteasome catalytic subunit 3; proteasome subunit, beta type, 5; PSX large multifunctional protease X; multicatalytic endopeptidase complex epsilon chain; Multicatalytic endopeptidase complex epsilon chain; Proteasome epsilon chain; Proteasome subunit X; Proteasome chain 6; Proteasome subunit MB1; Macropain epsilon chain
• Gene ID: 5693
• mRNA Refseq: NM_001130725
• Protein Refseq: NP_001124197
• MIM: 600306
• UniProt ID: P28074
• Chromosome Location: 14q11.2
• Function: peptidase activity;' protein binding; threonine-type endopeptidase activity