Recombinant Human PRPH Protein, His-tagged

• Cat.No.: PRPH-2627H
• Product Overview: Recombinant Human PRPH Protein (Met1-Tyr470) with a N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Met1-Tyr470
• Description: This gene encodes a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 57.4 kDa
  Accurate Molecular Mass: 58 kDa
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PRPH peripherin [ Homo sapiens (human) ]
• Official Symbol: PRPH
• Synonyms: PRPH; peripherin; NEF4; PRPH1; neurofilament 4 (57kD)
• Gene ID: 5630
• mRNA Refseq: NM_006262
• Protein Refseq: NP_006253
• MIM: 170710
• UniProt ID: P41219