Recombinant Human Protein Phosphatase 5, Catalytic Subunit

• Cat.No.: PPP5C-177H
• Product Overview: Recombinant human PPP5C (Serine/Threonine-Protein Phospatase) cloned from human cDNA with a N-terminal purification tag was expressed inE. coli. It consists of the mature PP5 (residues 16-499, Accession number: P53041). The N-terminal tag is cleaved during purification. MW = 56.8 KDa.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Serine/threonine-protein phosphatase 5 is an enzyme that in humans is encoded by the PPP5C gene. PPP5C has been shown to interact with ASK1, CRY2and GNA12.
• Purity: > 95% by SDS-PAGE. The protein was observed as a single band migrating at amolecular weight between 45 and 66KDa.
• Supplied As: 1 mg/mlin 50mM Tris-HCl pH 7.5, 150mM NaCl, 1mM DTT (dithiothreitol). The concentration is calculated from the absorbance at 280nm (e280= 57355 M-1 cm-1).
• Characteristics: Under the above described conditions, to avoid precipitation, the product can be concentrated to a maximum of 1mM.
• Storage: -20ºC. The protein is stable at 4ºC for at least 2 weeks and at 25ºC for at least several days. After initial defrost, aliquot product into individual tubes and refreeze at -20ºC. Avoid repeated freeze/defrost cycles.

Gene Information

• Gene Name: PPP5C protein phosphatase 5, catalytic subunit [ Homo sapiens ]
• Synonyms: PPP5C; protein phosphatase 5, catalytic subunit; PP5; PPP5; FLJ36922; Serine/Threonine phosphatase; EC 3.1.3.16; Serine/threonine-protein phosphatase 5; Protein phosphatase T; PP-T; PPT; FLJ36922
• Gene ID: 5536
• mRNA Refseq: NM_006247
• Protein Refseq: NP_006238
• MIM: 600658
• UniProt ID: P53041
• Chromosome Location: 19q13.3
• Pathway: MAPK signaling pathway
• Function: hydrolase activity; iron ion binding; manganese ion binding; metal ion binding; protein domain specific binding; protein serine/threonine phosphatase activity; signal transducer activity