Active Recombinant Full Length Human protein kinase C, Alpha, His-tagged

• Cat.No.: PRKCA-1301H
• Product Overview: Therecombinant human PRKCA was expressed in E. coli and purified by Ni-agarose chromatography.It is suitable for labeling PKC substrates.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-672 a.a.
• Description: PRKCA isa serine/threonine kinase and a member of the conventional (classical) PKCs(cPKCs), which have four conserved (C1 to C4) regions. This ubiquitouslyexpressed PKC isotype is activated in response to many different kinds ofstimuli and translocates from cytosol to the specialized cellularcompartments (nucleus, focal adhesion, caveolae, etc.) where it is presumedto work. Therefore, PRKCA has been implicated in a variety of cellularfunctions including proliferation, apoptosis, differentiation, motility, andinflammation.
• Form: Liquid ina 20 mM NaH2PO4 buffer, pH 7.8, + 500 mM NaCl + 1 mM Na-orthovanadate + 5 mMNaF + 40 mM β-glycerophosphate + 5% glycerol.
• Molecular Weight: 81.9 kDa
• Purity: > 95% by SDS-PAGE
• Specific Activity: >200,000 U/mg (1 Unit is defined as 1 picomole phosphate transferred to PKCpeptide QKRPSQRSKYL per min at 30°C).
• Endotoxin Level: < 0.1ng/μg of protein.
• Storage: For longterm storage, aliquot and freeze at -80°C. Stable for 1 year. Avoid repeated freeze-thawcycles.
• OfficialSymbol: PRKCA
• Function: nucleotide binding; proteinbinding; protein kinase activity; protein serine/threonine kinase activity; zincion binding; metal ion binding; histone kinase activity (H3-T6 specific); enzymebinding; ATP binding

Gene Information

• Gene Name: PRKCA protein kinase C, alpha [ Homosapiens ]
• Synonyms: PRKCA; proteinkinase C, alpha; AAG6; PKCA; PRKACA; MGC129900; MGC129901; PKC-alpha; proteinkinase C, alpha type; PKC-A; aging-associated gene 6; EC2.7.11.13
• Gene ID: 5578
• mRNA Refseq: NM_002737
• Protein Refseq: NP_002728
• MIM: 176960
• UniProt ID: P17252
• Chromosome Location: 17q22-q24
• Pathway: ATF-2 transcription factornetwork; African trypanosomiasis; Aldosterone-regulated sodium reabsorption; Alpha6-Beta4Integrin Signaling Pathway; Amoebiasis; Ca-dependent events; CaM pathway; CalciumRegulation in the Cardiac Cell; Calcium signaling pathway; Calmodulin inducedevents; Canonical NF-kappaB pathway; Cholinergic synapse; DAG and IP3signaling; Disinhibition of SNARE formation; Dopaminergic synapse