Recombinant Human Proteasome (prosome, macropain) Subunit, Beta Type, 2, His-tagged

• Cat.No.: PSMB2-1321H
• Product Overview: Recombinant human PSMB2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: PSMB2 is a member of the proteasome T1B family, which is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 30% glycerol, 0.1 M NaCl.
• Molecular Weight: 24.9 kDa (221 aa) confirmed by MALDI-TOF
• Purity: >90 % by SDS-PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MEYLIGIQGP DYVLVASDRV AASNIVQMKD DHDKMFKMSE KILLLCVGEA GDTVQFAEYI QKNVQLYKMR NGYELSPTAA ANFTRRNLAD CLRSRTPYHV NLLLAGYDEH EGPALYYMDY LAALAKAPFA AHGYGAFLTL SILDRYYTPT ISRERAVELL RKCLEELQKR FILNLPTFSV RIIDKNGIHD LDNISFPKQG S
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: APC/C-mediated degradation of cell cycle proteins; Adaptive Immunity Signaling; Antigen processing; Apoptosis; Assembly of the pre-replicative complex; Autodegradation of the E3 ubiquitin ligase COP1; Cell Cycle Checkpoints; DNA Replication; G1/S DNA Damage Checkpoints; HIV Infection; G1/S Transition; Immune System; Immunoproteasome; Metabolism of RNA

Gene Information

• Gene Name: PSMB2 proteasome (prosome, macropain) subunit, beta type, 2 [ Homo sapiens ]
• Official Symbol: PSMB2
• Synonyms: PSMB2; proteasome (prosome, macropain) subunit, beta type, 2; HC7-I; MGC104215; MGC126885; proteasome subunit beta type-2; OTTHUMP00000004345; macropain subunit C7-I; proteasome beta 2 subunit; proteasome component C7-I; proteasome subunit, beta type, 2; multicatalytic endopeptidase complex subunit C7-1; multicatalytic endopeptidase complex subunit C7-I; NP_001186708.1; EC 3.4.25.1
• Gene ID: 5690
• mRNA Refseq: NM_001199779
• Protein Refseq: NP_001186708
• MIM: 602175
• UniProt ID: Q59FJ0
• Chromosome Location: 1p34.2
• Function: peptidase activity; threonine-type endopeptidase activity