Recombinant Human Proteasome (prosome, macropain) Subunit, Alpha Type, 4, His-tagged

• Cat.No.: PSMA4-1565H
• Product Overview: Recombinant human PSMA4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: PSMA4, also known as proteasome subunit alpha type 4, is a member of the peptidase T1A family that is a 20S core alpha subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. It is distributed throughout eukaryotic cells at a high concentration and cleaves peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 20% glycerol, 1mM DTT, 0.1mM PMSF.
• Molecular Weight: 32.0 kDa (285aa), confirmed by MALDI-TOF.
• Purity: >95% by SDS-PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMSRRYD SRTTIFSPEG RLYQVEYAME AIGHAGTCLG ILANDGVLLA AERRNIHKLL DEVFFSEKIYKLNEDMACSV AGITSDANVL TNELRLIAQR YLLQYQEPIP CEQLVTALCD IKQAYTQFGG KRPFGVSLLY IGWDKHYGFQ LYQSDPSGNYGGWKATCIGN NSAAAVSMLK QDYKEGEMTL KSALALAIKV LNKTMDVSKL SAEKVEIATL TRENGKTVIR VLKQKEVEQL IKKHEEEEAKAEREKKEKEQ KEKDK
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: APC/C-mediated degradation of cell cycle proteins; APC/C:Cdc20 mediated degradation of Securin; APC/C:Cdc20 mediated degradation of mitotic proteins; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins; Adaptive Immunity Signaling; Antigen processing: Ubiquitination& Proteasome degradation; Apoptosis; Assembly of the pre-replicative complex; Autodegradation of Cdh1 by Cdh1:APC/C; Autodegradation of the E3 ubiquitin ligase COP1; CDK-mediated phosphorylation and removal of Cdc6; CDT1 association with the CDC6:ORC:origin complex; Cell Cycle Checkpoints; Cell Cycle

Gene Information

• Gene Name: PSMA4 proteasome (prosome, macropain) subunit, alpha type, 4 [ Homo sapiens ]
• Official Symbol: PSMA4
• Synonyms: PSMA4; proteasome (prosome, macropain) subunit, alpha type, 4; HC9; PSC9; HsT17706; MGC12467; MGC24813; MGC111191; proteasome subunit alpha type-4; OTTHUMP00000185020; macropain subunit C9; proteasome subunit L; proteasome subunit HC9; proteasome component C9; multicatalyticendopeptidase complex subunit C9; EC 3.4.25.1; Macropain subunit C9; Multicatalyticendopeptidase complex subunit C9; Proteasome component C9
• Gene ID: 5685
• mRNA Refseq: NM_001102667
• Protein Refseq: NP_001096137
• MIM: 176846
• UniProt ID: P25789
• Chromosome Location: 15q25.1
• Function: peptidase activity; protein binding; threonine-type endopeptidase activity