Recombinant Human Proteasome (prosome, macropain) Subunit, Alpha Type, 2, His-tagged

• Cat.No.: PSMA2-6877H
• Product Overview: Recombinant human PSMA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 28.0 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-234aa
• Description: PSMA2, also known as proteasome subunit alpha type-2, is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. PSMA2, belonged to the peptidase T1A family, is a 20S core alpha subunit of proteasome. This protein serves as docking domains for the regulatory particles and exterior gates blocking unregulated access to the interior cavity.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 30% glycerol 0.1M NaCl,1mM DTT, 0.1mM PMSF.
• Molecular Mass: 28.0 kDa (254aa), confirmed by MALDI-TOF.
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MAERGYSFSL TTFSPSGKLV QIEYALAAVA GGAPSVGIKA ANGVVLATEK KQKSILYDER SVHKVEPITK HIGLVYSGMG PDYRVLVHRA RKLAQQYYLV YQEPIPTAQL VQRVASVMQE YTQSGGVRPF GVSLLICGWN EGRPYLFQSD PSGAYFAWKA TAMGKNYVNG KTFLEKRYNE DLELEDAIHT AILTLKESFE GQMTEDNIEV GICNEAGFRR LTPTEVKDYL AAIA
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: PSMA2 proteasome (prosome, macropain) subunit, alpha type, 2 [ Homo sapiens ]
• Official Symbol: PSMA2
• Synonyms: PSMA2; proteasome (prosome, macropain) subunit, alpha type, 2; proteasome subunit alpha type-2; HC3; MU; PMSA2; macropain subunit C3; proteasome subunit HC3; proteasome component C3; multicatalytic endopeptidase complex subunit C3; PSC2
• Gene ID: 5683
• mRNA Refseq: NM_002787
• Protein Refseq: NP_002778
• MIM: 176842
• UniProt ID: P25787
• Chromosome Location: 7p13
• Pathway: APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Securin, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; Activation of NF-kappaB in B Cells, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem
• Function: peptidase activity; protein binding; threonine-type endopeptidase activity