Recombinant Human PRKD2 protein, His & T7-tagged

• Cat.No.: PRKD2-2713H
• Product Overview: Recombinant Human PRKD2 aa. (Glu621~Ser832 (Accession # Q9BZL6)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 28.2kDa
• Protein length: Glu621~Ser832
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.7.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: PRKD2 protein kinase D2 [ Homo sapiens (human) ]
• Official Symbol: PRKD2
• Synonyms: PKD2; HSPC187; nPKC-D2; serine/threonine-protein kinase D2
• Gene ID: 25865
• mRNA Refseq: NM_001079880.1
• Protein Refseq: NP_001073349.1
• UniProt ID: Q9BZL6