Recombinant human PPP1R2, His-tagged

• Cat.No.: PPP1R2-2438H
• Product Overview: Recombinant human PPP1R2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: PPP1R2 is an inhibitor of protein-phosphatase 1 and forms heterodimer with PP1. Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex. Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex.
• Form: Liquid. In 20mM Tris-HCl(pH8.0) containing 30% glycerol, 0.15M NaCl, 1mM DTT
• Molecular Mass: 21kDa (189aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAASTAS HRPIKGILKN KTSTTSSMVA SAEQPRGNVD EELSKKSQKW DEMNILATYH PADKDYGLMKIDEPSTPYHS MMGDDEDACS DTEATEAMAP DILARKLAAA EGLEPKYRIQ EQESSGEEDS DLSPEEREKK RQFEMKRKLH YNEGLNIKLA RQLISKDLH
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: PPP1R2 protein phosphatase 1, regulatory (inhibitor) subunit 2 [ Homo sapiens ]
• Official Symbol: PPP1R2
• Synonyms: PPP1R2; protein phosphatase 1, regulatory (inhibitor) subunit 2; protein phosphatase inhibitor 2; IPP2; phosphatase inhibitor 2; phosphoprotein phosphatase; IPP-2; MGC87148
• Gene ID: 5504
• mRNA Refseq: NM_006241
• Protein Refseq: NP_006232
• MIM: 601792
• UniProt ID: P41236
• Chromosome Location: 3q29
• Function: protein binding; protein serine/threonine phosphatase inhibitor activity